The Science

This new method of using Nuclear Magnetic Resonance (NMR) to directly measure compounds such as Oleocanthal and Oleacein was revolutionary in the EVOO market. Up until the development of the NMR analysis, many of the EVOO health benefits were credited to the presence of monosaturated fat - as endorsed by the widely accepted Mediterranean Diet.

However, the NMR technique made it possible to measure and identify phenolic compounds that provide additional health benefits found only in high phenol EVOO. Scientific studies so far have suggested that oleocanthal acts as a natural anti-inflammatory agent that protects cells from the oxidation process (which leads to premature aging and disease). And while the benefits continue to be better understood, many studies have supported that Oleocanthal may even help fight against several cardiovascular and neurodegenerative diseases such as Alzheimer’s and even cancer.

The Nuclear Magnetic Resonance (NMR) method we use can accurately measure the phenolic compounds Oleocanthal and Oleacein, which are the two most abundant forms of conjugated hydroxytyrosol and tyrosol in most olive oils, as well as oleuropein aglycon and ligstroside aglycon. We continuously test our oil by a third party to guarantee quality and purity as well as to track the stability of the phenolic compounds. High Phenol EVOO has been shown to remain relatively stable over time. Each bottle of Olea Blue is labeled with the total phenolic compound range found at the time the oil is shipped.

Scientific Studies On The Health Benefits of High Phenol EVOO

Alaa H Abuznait · Hisham Qosa · Belnaser A Busnena · Khalid A El Sayed · Amal Kaddoumi

ABSTRACT: Oleocanthal, a phenolic component of extra-virgin olive oil, has been recently linked to reduced risk of Alzheimer’s disease (AD), a neurodegenerative disease that is characterized by accumulation of β-amyloid (Aβ) and tau proteins in the brain. However, the mechanism by which oleocanthal exerts its neuroprotective effect is still incompletely understood. Here, we provide in vitro and in vivo evidence for the potential of oleocanthal to enhance Aβ clearance from the brain via up-regulation of P-glycoprotein (P-gp) and LDL lipoprotein receptor related protein-1 (LRP1), major Aβ transport proteins, at the blood-brain barrier (BBB). Results from in vitro and in vivo studies demonstrated similar and consistent pattern of oleocanthal in controlling Aβ levels. In cultured mice brain endothelial cells, oleocanthal treatment increased P-gp and LRP1 expression and activity. Brain efflux index (BEI%) studies of (125)I-Aβ(40) showed that administration of oleocanthal extracted from extra-virgin olive oil to C57BL/6 wild-type mice enhanced (125)I-Aβ(40) clearance from the brain and increased the BEI% from 62.0 ± 3.0% for control mice to 79.9 ± 1.6% for oleocanthal treated mice. Increased P-gp and LRP1 expression in the brain microvessels and inhibition studies confirmed the role of up-regulation of these proteins in enhancing (125)I-Aβ(40) clearance after oleocanthal treatment. Furthermore, our results demonstrated significant increase in (125)I-Aβ(40) degradation as a result of the up-regulation of Aβ degrading enzymes following oleocanthal treatment. In conclusion, these findings provide experimental support that potential reduced risk of AD associated with extra-virgin olive oil could be mediated by enhancement of Aβ clearance from the brain.

Feb 2013 · ACS Chemical Neuroscience

Lisa Parkinson · Russell Keast

ABSTRACT: Virgin olive oil (VOO) is credited as being one of many healthful components of the Mediterranean diet. Mediterranean populations experience reduced incidence of chronic inflammatory disease states and VOO is readily consumed as part of an everyday dietary pattern. A phenolic compound contained in VOO, named oleocanthal, shares unique perceptual and anti-inflammatory characteristics with Ibuprofen. Over recent years oleocanthal has become a compound of interest in the search for naturally occurring compounds with pharmacological qualities. Subsequent to its discovery and identification, oleocanthal has been reported to exhibit various modes of action in reducing inflammatory related disease, including joint-degenerative disease, neuro-degenerative disease and specific cancers. Therefore, it is postulated that long term consumption of VOO containing oleocanthal may contribute to the health benefits associated with the Mediterranean dietary pattern. The following paper summarizes the current literature on oleocanthal, in terms of its sensory and pharmacological properties, and also discusses the beneficial, health promoting activities of oleocanthal, in the context of the molecular mechanisms within various models of disease. Keywords: virgin olive oil; ibuprofen; sensory; inflammation; health

Jul 2014 · International Journal of Molecular Sciences

ABSTRACT: We explored the anti-cancer capacity of (-)-oleocanthal in human hepatocellular carcinoma (HCC). (-)-Oleocanthal inhibited proliferation and cell cycle progression and induced apoptosis in HCC cells in vitro and suppressed tumor growth in an orthotopic HCC model. (-)-Oleocanthal also inhibited HCC cell migration and invasion in vitro and impeded HCC metastasis in an in vivo lung metastasis model. ( )-Oleocanthal acted by inhibiting epithelial-mesenchymal transition (EMT) through downregulation Twist, which is a direct target of STAT3. (-)-Oleocanthal also reduced STAT3 nuclear translocation and DNA binding activity, ultimately downregulating its downstream effectors, including the cell cycle protein Cyclin D1, the anti-apoptotic proteins Bcl-2 and survivin, and the invasion-related protein MMP 2. Overexpression of constitutively active STAT3 partly reversed the anti cancer effects of (-)-oleocanthal, which inhibited STAT3 activation by decreasing the activities of JAK1 and JAK2 and increasing the activity of SHP-1. These data suggest that (-)-oleocanthal may be a promising candidate for HCC treatment.·

Jun 2016 · Oncotarget

Article: (-)-Oleocanthal inhibits growth and metastasis by blocking activation of STAT3 in human hepatocellular carcinomaTiemin Pei · Qinghui Meng · Jihua Han · Haobo Sun Long Li ·Ruipeng Song · Boshi Sun · Shangha Pan · Desen Liang · Lianxin Liu

ABSTRACT: Oleocanthal is one of the phenolic compounds of extra virgin olive oil with important anti-inflammatory properties. Although its potential anticancer activity has been reported, only limited evidence has been provided in cutaneous malignant melanoma. The present study is aimed at investigating the selective in vitro antiproliferative activity of oleocanthal against human malignant melanoma cells. Since oleocanthal is not commercially available, it was obtained as a pure standard by direct extraction and purification from extra virgin olive oil. Cell viability experiments carried out by WST-1 assay demonstrated that oleocanthal had a remarkable and selective activity for human melanoma cells versus normal dermal fibroblasts with IC50s in the low micromolar range of concentrations. Such an effect was paralleled by a significant inhibition of ERK1/2 and AKT phosphorylation and downregulation of Bcl-2 expression. These findings may suggest that extra virgin olive oil phenolic extract enriched in oleocanthal deserves further investigation in skin cancer.

Jun 2016 · Nutrition and Cancer

Article: Cytotoxic Activity of Oleocanthal Isolated from Virgin Olive Oil on Human Melanoma CellsStefano Fogli · Chiara Arena · Sara Carpi · Beatrice Polini · Simone Bertini · Maria Digiacomo · Francesca Gado · Alessandro Saba ·Giuseppe Saccomanni · Maria Cristina Breschi · Paola Nieri ·Clementina Manera · Marco Macchia

ABSTRACT: Adherence to the Mediterranean Diet (MD) has been associated with a reduced incidence of neurodegenerative diseases and better cognitive performance. Virgin olive oil, the main source of lipids in the MD, is rich in minor phenolic components, particularly hydroxytyrosol (HT). HT potent antioxidant and anti-inflammatory actions have attracted researchers’ attention and may contribute to neuroprotective effects credited to MD. In this review HT bioavailability and pharmacokinetics are presented prior to discussing health beneficial effects. In vitro and in vivo neuroprotective effects together with its multiple mechanisms of action are reviewed. Other microconstituents of olive oil are also considered due to their potential neuroprotective effects (oleocanthal, triterpenic acids). Finally, we discuss the potential role of HT as a therapeutic tool in the prevention of neurodegenerative diseases.

Mar 2015 · Molecules

Article: Potential Role of Olive Oil Phenolic Compounds in the Prevention of Neurodegenerative DiseasesJose Rodríguez-Morató · Laura Xicota · Montse Fitó · Magí Farré ·Mara Dierssen · Rafael de la Torre


ABSTRACT: Background: Oleacein (dialdehydic form of decarboxymethyl elenolic acid linked to hydroxytyrosol; 3,4-DHPEA-EDA) have been proven to possess antioxidant and anti-inflammatory activity. Purpose: In this study, we examined whether oleacein could increase CD163 and IL-10 receptor expression as well as HO-1 intracellular secretion in human macrophages. Methods: Effect of oleacein (10 and 20 μmol/l) or oleacein together with complexes of haemoglobin (Hb) and haptoglobin 1-1 (Hp11) or haptoglobin 2-2 (Hp22) on expression of IL-10 and CD163 receptor was determined by Flow Cytometry. Expression of CD163mRNA was measured by real-time quantitative RT-PCR. Heme oxygenase 1 (HO-1) intracellular secretion in macrophages was investigated by enzyme-linked immunosorbent assay (ELISA). Results: Oleacein (OC) together with complexes HbHp11 or HbHp22 stimulated the expression of CD163 (30-100-fold), IL-10 (170-300-fold) and HO-1 secretion (60-130-fold) after 5 days of coincubation. The 2-fold (24 h), 4-fold (48 h) increase of CD163 mRNA level and its final (72 h) decrease was also observed. Conclusion: Our results suggested that oleacein enhances anti-inflammatory activity of complexes haemoglobin with haptoglobin 1-1 and 2-2 and could play a potential role in the prevention of inflammatory disease related to atherosclerosis.

Nov 2015 · Phytomedicine: international journal of phytotherapy and phytopharmacology

Article: Oleacein enhances anti-inflammatory activity of human macrophages by increase CD163 receptor expressionAgnieszka Filipek · Monika E. Czerwińska · Anna K. Kiss ·Małgorzata Wrzosek · Marek Naruszewicz

ABSTRACT: Oleocanthal is a bioactive compound from olive oil. It has attracted considerable attention as it is anti-inflammatory, antiproliferative, and has been shown to possess neuroprotective properties in vitro and in vivo. Delineated from its polyphenolic structure, the aim of this study was to characterize oleocanthal towards estrogenic properties. This might contribute to partly explain the beneficial effects described for the Mediterranean diet. Estrogenic properties of oleocanthal were assessed by different methods: a) stimulation of reporter gene activity in MVLN or RNDA cells either expressing estrogen receptor α or β, b) stimulation of luciferase reporter gene activity in U2OS osteosarcoma cells expressing estrogen receptor α or β, and c) elucidation of the impact on estradiol-induced gene expression in U2OS cells transduced with both estrogen receptors. Depending on the cell line origin, oleocanthal inhibited luciferase activity (MVLN, U2OS-estrogen receptor β) or weakly induced reporter gene activity at 10 µM in U2OS-estrogen receptor α cells. However, oleocanthal inhibited stimulation of luciferase activity by estradiol from both estrogen receptors. Oleocanthal, if given alone, did not stimulate gene expression in U2OS cells, but it significantly modulated the response of estradiol. Oleocanthal enhanced the effect of estradiol on the regulation of those genes, which are believed to be regulated through heterodimeric estrogen receptors. As the estrogenic response pattern of oleocanthal is rather unique, we compared the results obtained with oleacein. Oleocanthal binds to both estrogen receptors inducing estradiol-agonistic or antiagonistic effects depending on the cell line. Regarding regulation of gene expression in U2OS-estrogen receptor α/β cells, oleocanthal and oleacein enhanced estradiol-mediated regulation of heterodimer-regulated genes.

Jul 2015 · Planta Medica

Article: Oleocanthal Modulates Estradiol-Induced Gene Expression Involving Estrogen ReceptorAnnekathrin Martina Keiler · Sefirin Djiogue · Tino Ehrhardt · Oliver Zierau · Leandros Skaltsounis · Maria Halabalaki · Günter Vollmer